Why can the oriscience luminescence cell viability assay kit complete the test in just 10 minutes?
Publish Time: 2025-05-15
The oriscience luminescence cell viability assay kit uses chemiluminescence to determine the intracellular ATP content, thereby achieving ultra-high sensitivity and ultra-wide linear range of live cell number quantitative detection. This kit is not only easy to operate, but also takes only 10 minutes from adding reagents to completing the test.1. Efficient cell lysis solutionThe kit uses an optimized cell lysis solution, which can quickly and completely destroy the cell membrane and release all components in the cell, including ATP. Traditional cell lysis methods may require multiple washing, centrifugation and other steps, which takes a long time. The optimized lysis solution can complete cell lysis in a few minutes with simple addition, greatly shortening the processing time.Rapid lysis: The optimized lysis solution can effectively lyse various types of cells (such as adherent cells and suspension cells) in a short time, ensuring rapid release of ATP.Gentle but efficient: Despite the fast lysis speed, the lysis solution has minimal effect on ATP and will not cause ATP degradation or inactivation, ensuring the accuracy of the test results.2. Highly active and stable firefly luciferaseThe firefly luciferase modified by enzyme evolution is used in the kit. This enzyme has higher catalytic efficiency and stability, and can convert ATP into visible chemiluminescent signals in a short time.High catalytic efficiency: The genetically engineered luciferase has higher specific activity and can catalyze the reaction of ATP and luciferin to generate a large number of photons in a very short time, thereby generating a strong and stable luminescent signal.Thermal stability and anti-interference ability: The improved luciferase has better thermal stability and anti-interference ability, and can maintain efficient catalysis even in complex cell culture environments, further improving the detection speed and reliability.3. Optimized buffer systemIn addition to the efficient lysis buffer, the kit is also equipped with a carefully designed buffer system, which can maintain the optimal reaction conditions and ensure rapid chemiluminescent reactions.pH and ionic strength control: The buffer can accurately adjust the pH and ionic strength of the reaction environment, provide ideal catalytic conditions for luciferase, and promote rapid reactions between ATP and luciferin.Inhibitor removal: Some cell culture media contain components that inhibit ATP detection. The optimized buffer can effectively remove these inhibitors and avoid their influence on the reaction rate.4. No need to wash or replace the culture mediumSome traditional cell viability detection methods (such as MTT, CCK-8, etc.) usually require multiple washing or replacement of the culture medium before detection, which not only increases the complexity of the operation, but also prolongs the time of the entire experiment. In contrast, the operation process of the oriscience luminescence cell viability assay kit is extremely simplified.Direct sample addition detection: Users only need to add the lysate and luciferase reagent directly to the culture dish or well plate containing cells without any additional washing or replacement steps, which greatly saves time and labor.Reduce operational errors: By eliminating multiple steps, the errors caused by human operation are reduced, and the consistency and repeatability of experimental results are improved.5. Quick signal readingOnce the chemiluminescent reaction is started, the light signal generated can be immediately captured by a highly sensitive photometer or microplate reader, with almost no delay.Real-time monitoring: The instrument can record the intensity of the light signal within seconds, which means that the time from adding reagents to obtaining preliminary results is very short.Automation support: Many modern laboratory equipment supports automated operations, which further speeds up data acquisition and makes the entire detection process more efficient.In summary, the reason why the oriscience luminescence cell viability assay kit can complete the detection in just 10 minutes is mainly due to its efficient cell lysis solution, highly active and stable firefly luciferase, optimized buffer system and simplified operation process. These features work together to not only greatly shorten the detection time, but also improve the sensitivity and accuracy of the detection, making it a highly competitive cell viability detection tool.
