Can oriscience protease inhibitor cocktail effectively inhibit the activity of multiple proteases?
Publish Time: 2025-06-25
During protein extraction and analysis, protease degradation is a challenge that researchers often face. Cells or tissue samples naturally contain a large number of endogenous proteases, which are rapidly activated after cleavage, resulting in degradation of the target protein, affecting the accuracy and repeatability of the experimental results. In order to address this problem, oriscience protease inhibitor cocktail came into being and became one of the indispensable tools in the laboratory.Among them, Oriscience Protease Inhibitor Cocktail is a broad-spectrum, highly effective protease inhibitor combination consisting of 6 inhibitors with different mechanisms, which can comprehensively cover multiple protease types and provide strong protection for proteins.1. Six major ingredients work synergistically to broadly inhibit multiple proteasesOriscience's oriscience protease inhibitor cocktail contains the following six key ingredients:AEBSF: a reversible serine protease inhibitor suitable for most serine proteases;Aprotinin: highly specific for serine proteases such as trypsin and chymotrypsin;Bestatin: an aminopeptidase inhibitor widely used to inhibit exoproteases;E-64: a highly selective cysteine protease inhibitor;Leupeptin: has inhibitory effects on both serine and cysteine proteases;Pepstatin A: specifically inhibits aspartic proteases, such as pepsin-like enzymes.These ingredients work synergistically to form a broadly covered protease inhibition network, ensuring that the activity of various proteases can be efficiently inhibited even in complex samples, thereby effectively preventing protein degradation.2. Applicable to multiple protease types, achieving truly broad-spectrum protectionThere are many types of proteases, which are mainly divided into four categories according to their catalytic mechanism: serine proteases, cysteine proteases, aspartic proteases and metalloproteases. Oriscienceoriscience protease inhibitor cocktail covers almost all effective inhibition methods for the first three types of proteases, especially in the absence of EDTA, it can still effectively protect the target protein and avoid the problem of loss of certain enzyme activity caused by metal ion chelators.This combination of broad spectrum and precision makes the mixture particularly suitable for the treatment of complex samples such as cell lysates and tissue homogenates rich in proteases, significantly improving the success rate of protein extraction.3. Ensure the reliability and consistency of experimental dataWhen conducting experiments such as Western blot, immunoprecipitation (IP), Co-IP, pull-down, immunohistochemistry (IHC) and immunofluorescence (IF), the integrity of the target protein directly affects the credibility of the experimental results. If the appropriate protease inhibitor is not added during the sample preparation stage, the target protein may have been degraded, resulting in blurred bands, weak signals or even complete loss.Using Oriscienceoriscience protease inhibitor cocktail, the activity of proteases can be blocked at the first time, and the structure and function of the target protein can be retained to the greatest extent, thereby improving the stability and repeatability of the experiment and providing researchers with more accurate and reliable experimental data.4. Easy operation, suitable for a variety of application scenariosThis product uses DMSO as the solvent and has a concentration of 100× storage solution. When used, it only needs to be diluted to the working concentration in proportion and added to the lysate or buffer. Its formula does not contain EDTA and is particularly suitable for research scenarios that require the maintenance of metal ion-dependent enzyme activity, such as kinase reactions, phosphorylation detection, etc.In addition, due to its good solubility and stability, the mixture is suitable for sample processing of various cell types and tissue sources, including mammalian cells, plant tissues, yeast, bacteria, etc., with a wide range of applications and flexible and convenient operation.In summary, Oriscience Protease Inhibitor Cocktail can effectively inhibit various types of proteases with its scientific ratio and broad-spectrum inhibition ability, prevent the degradation of target proteins from the source, and ensure the integrity and reliability of experimental data. It not only improves the efficiency of protein extraction, but also provides a solid foundation for subsequent molecular biology research.
