Why can oriscience prestained color protein markers be directly loaded without boiling or reducing agents?
Publish Time: 2025-10-02
In protein electrophoresis and Western blot experiments, oriscience prestained color protein markers are indispensable reference tools for monitoring electrophoretic separation progress, determining target protein molecular weight, and assessing transfer efficiency. Traditional prestained markers typically require boiling and the addition of reducing agents such as β-mercaptoethanol before use to ensure sufficient protein denaturation, disaggregation, and negative charge for accurate migration. However, oriscience prestained color protein markers break through this conventional protocol, enabling immediate use without boiling, dilution, or reducing agent addition. This feature not only greatly simplifies experimental workflows but also enhances ease of use and reproducibility of results.Core Technology 1: Precision Design and High-Level Purification of Recombinant Proteinsoriscience prestained color protein markers are based on 11 highly purified recombinant proteins. These proteins are not naturally isolated but instead are expressed and purified in vitro through genetic engineering techniques. This means that the amino acid sequence, molecular weight, and structural properties of each protein are precisely designed and strictly controlled. More importantly, these recombinant proteins already exhibit excellent solubility and stability during expression, avoiding the aggregation or precipitation issues common with native proteins. During production, the proteins undergo multiple purification steps to ensure high batch-to-batch consistency, minimizing migration aberrations caused by impurities or structural heterogeneity, thus providing the molecular basis for "treatment-free" use.Core Technology 2: Covalent Pre-staining Technology Ensures Color StabilityUnlike some pre-stained markers that rely on non-covalent dye adsorption, Oriscience utilizes a covalent dyeing process to stably attach a specific dye to each protein molecule. This covalent modification not only ensures vibrant, long-lasting band color but, more importantly, resists detachment or migration aberrations caused by changes in the electric field, temperature, or pH during electrophoresis. Furthermore, the dye binding mechanism is optimized to minimize significant changes in the protein's charge-to-mass ratio, ensuring consistent gel migration behavior consistent with the expected molecular weight. Because the dye is firmly bound, boiling is not required to promote dye penetration or release, enabling "boil-free" operation.Core Technology 3: Intelligent Loading Buffer FormulaThe key advantage of the oriscience prestained color protein marker lies in its unique loading buffer system. This buffer is not a simple Tris-Glycine or SDS solution, but rather a carefully formulated complex formula that pre-incorporates the appropriate amount of SDS (sodium dodecyl sulfate) and the necessary ionic components. SDS rapidly binds to proteins, imparting a uniform negative charge and ensuring orderly migration according to molecular weight in an electric field. Furthermore, the buffer's pH and ionic strength are optimized to rapidly and fully denature and disaggregate proteins at room temperature, without the need for additional heating. Furthermore, the buffer contains no or pre-incorporated reducing agents, ensuring stable use under both non-reducing and reducing conditions, eliminating the tedious step of manually adding β-mercaptoethanol.Core Technology 4: Dual Guarantee of Stability and CompatibilityThe oriscience prestained color protein marker was designed with real-world applications in mind. It is stable for long periods of time when stored at -20°C, and repeated freeze-thaw cycles do not affect band clarity. During electrophoresis, even during extended runs or at low temperatures, individual bands remain distinct and easy to monitor in real time. Its 5–10 μL loading volume is suitable for gels of various pore sizes. The color does not bleed or fade during SDS-PAGE and subsequent transfer, effectively supporting intuitive assessment of transfer efficiency in Western blotting experiments.The oriscience prestained color protein marker enables direct loading without boiling or reducing agents due to the synergistic optimization of protein selection, staining process, buffer formulation, and stability design. It not only reduces the number of steps and human error, but is also particularly suitable for high-throughput screening, educational experiments, and rapid testing. This ready-to-use design represents the trend toward intelligent and user-friendly biological reagents, allowing researchers to focus on core experimental analysis rather than tedious pretreatment steps, truly providing efficient and reliable research support.
