How does the mouse liver tissue rapid dissociation kit efficiently obtain highly active single-cell suspensions within 30 minutes?
Publish Time: 2026-01-07
In modern life science research, single-cell level analysis has become a key tool for revealing liver development, metabolic regulation, immune responses, and disease mechanisms. However, the liver, as a highly vascularized organ rich in connective tissue and extracellular matrix, has a dense tissue structure and complex cell types. Traditional dissociation methods are often time-consuming, inefficient, and prone to cell damage or phenotypic changes. Efficient, gentle, and rapid acquisition of highly active, high-purity single-cell suspensions is a prerequisite for downstream experiments such as single-cell sequencing, flow cytometry, and primary culture. The mouse liver tissue rapid dissociation kit is designed to address this challenge. With its specially formulated enzymatic digestion system and optimized process, it can prepare high-quality single-cell suspensions within 30 minutes.1. Specially Formulated Complex Enzyme: Precisely Targeting Liver ECMThe core advantage of this kit lies in its specially formulated dissociation enzyme formula developed specifically for liver tissue. This formula is not a single collagenase, but a scientifically formulated blend of high-purity collagenase IV, neutral protease, hyaluronidase, and a small amount of elastase. While preserving the integrity of cell surface antigens, it synergistically degrades major extracellular matrix components in liver tissue—such as type I/IV collagen, fibronectin, laminin, and hyaluronic acid. This multi-enzyme synergy significantly improves tissue dissociation efficiency, avoiding the problems of incomplete action or over-digestion associated with single enzymes, ensuring the gentle release of cells from the matrix.2. 30-Minute Rapid Process: Balancing Speed and Cell ViabilityTraditional liver tissue dissociation often requires 60–90 minutes or even longer. Prolonged incubation can easily induce cell stress, apoptosis, or membrane protein degradation. This kit optimizes enzyme concentration, buffer pH, and incubation temperature, compressing the effective dissociation time to within 30 minutes. During this process, cells maintain high metabolic activity, with measured viability generally exceeding 90%. Simultaneously, the included stop solution rapidly inhibits enzyme activity, preventing over-digestion and further ensuring cell integrity.3. Simple Operation, Suitable for Micro-samplesThe experimental procedure is highly simplified: Fresh mouse liver tissue is simply cut into 1–2 mm³ pieces, added to preheated dissociation working solution, gently incubated at 37°C with gentle shaking for 20–30 minutes, followed by pipetting or gentle grinding, and then filtered through a 40–70 μm cell sieve to obtain a clear single-cell suspension. The entire process requires no complex equipment or additional reagents such as serum or DNase. It is particularly suitable for small amounts of liver tissue, minimizing sample waste.4. Compatible with Multiple Downstream Applications, High Data ReliabilityThe resulting single-cell suspension not only has high cell viability and low fragmentation, but also stable expression of cell surface markers, fully meeting the requirements for flow cytometry sorting and phenotypic analysis. Hepatocytes maintain intact morphology and strong adhesion, making them suitable for primary hepatocyte culture and functional experiments. More importantly, the RNA has high integrity and no significant degradation, providing high-quality starting material for single-cell RNA sequencing, ensuring that transcriptome data accurately reflects the in vivo state.5. Batch Stability and Experimental Reproducibility GuaranteeThe kit uses strictly quality-controlled GMP-grade enzyme raw materials, and each batch undergoes functional validation to ensure consistent dissociation effects. Researchers do not need to screen enzyme batches or optimize conditions themselves, significantly reducing experimental variability and improving research reproducibility.The mouse liver tissue rapid dissociation kit successfully overcomes the technical bottleneck of liver single-cell preparation through a three-pronged design of "dedicated formulation + rapid process + high compatibility." It not only saves valuable time but also lays a solid foundation for subsequent high-precision experiments with high cell viability and integrity, helping researchers achieve more reliable and in-depth research results in areas such as liver disease mechanisms, immune microenvironment, and regenerative medicine.
