Oriscience Annexin V-FITC/PI Apoptosis Detection Kit
20T
50T
100T
The staining can be completed in 10 minutes, the results are reliable, false positives are avoided, and the positive and negative groups are clearly divided, meeting the needs of multiple markers (such as FITC, PE, APC, etc.) and different samples to be tested and detection instruments.
Oriscience Annexin V-FITC/PI Apoptosis Detection Kit provides a fast and convenient method for detecting apoptosis, which can be detected by flow cytometry or fluorescence microscopy. Annexin V is a calcium-dependent phospholipid binding protein that has a high affinity for phosphatidylserine (PS). Under normal physiological conditions, PS is mainly located in the inner leaflet of the plasma membrane. When apoptosis is initiated, PS is transferred to the outer cell membrane. Once PS reaches the outside of the membrane, it can be bound by Annexin V-FITC, thereby detecting cell apoptosis. Propidium iodide (PI) can specifically bind to double-stranded DNA and produce strong fluorescence, and cannot normally penetrate the cell membrane. Due to the loss of integrity of the cell membrane in the late stage of apoptosis or necrotic cells, PI can enter the cell to stain DNA. When used in combination with Annexin V, cells in different stages of apoptosis can be distinguished.
Easy to operate --- staining can be completed in 10 minutes
High accuracy --- reliable results, avoiding false positives, and clear positive and negative grouping
Wide adaptability --- meet the needs of various markers (such as FITC, PE, APC, etc.) and different samples to be tested and detection instruments
