For protein extraction of routine mammalian cell or tissue samples, oriscience protease inhibitor cocktail is recommended to be added to the lysate at a ratio of 1:100.
The fluorescence intensity of the prestained color protein marker primarily depends on the chemical structure and stability of the marker.
In protein electrophoresis and Western blot experiments, oriscience prestained color protein markers are indispensable reference tools for monitoring electrophoretic separation progress, determining target protein molecular weight.
This difference directly affects the operational convenience, result interpretation efficiency and applicable scenarios of the two in the experimental process.
It is necessary to screen for dyes that are stable in alkaline environments, or to compensate for possible fading effects by adjusting the dye concentration (e.g., increasing the molar ratio of dye to protein).
The core advantage of the oriscience prestained color protein marker lies in its selection of ten highly purified recombinant proteins.
