How does the oriscience prestained color protein marker avoid nonspecific banding and improve experimental reliability?
Publish Time: 2025-08-21
In protein research, SDS-PAGE electrophoresis and Western blotting are fundamental and critical experimental techniques. Protein molecular weight markers, acting as "rulers" in experiments, not only determine the molecular weight of target proteins but also play a crucial role in monitoring electrophoretic separation and transfer efficiency. However, many researchers often encounter a vexing problem: nonspecific banding, tailing, smearing, or background interference from markers, making result interpretation difficult and even impacting experimental reliability. The oriscience prestained color protein marker is designed to address this pain point. With its highly purified, well-defined structure, and optimized preparation process, it significantly reduces nonspecific signal, providing a clear, reliable, and reproducible reference standard for scientific research.1. Highly Purified Recombinant Proteins: Eliminate Impurity Interference at the SourceThe core advantage of the oriscience prestained color protein marker lies in its selection of ten highly purified recombinant proteins. Each band is genetically engineered and rigorously purified by chromatography to ensure a single, structurally intact protein free of contaminants. Compared to traditional markers derived from native tissues, recombinant proteins avoid extraneous bands due to residual host cell proteins or degradation products. This "pure starting point" fundamentally eliminates the possibility of nonspecific migration or cross-reactions, resulting in sharp, well-defined pre-stained bands, significantly enhancing the readability and professionalism of the electrophoresis pattern.2. Pre-stained Covalent Binding: Dye Stability, No Shedding, No InterferenceThis marker utilizes covalent coupling technology to stably bind the dye to the protein molecule, rather than simple physical adsorption. Therefore, during electrophoresis, the dye will not shed or migrate due to changes in voltage, buffer composition, or temperature, ensuring consistent band color and precise positioning. The 72 kDa band is a distinctive orange-red, the 10 kDa band is green, and the remaining bands are blue. This tri-color design not only facilitates real-time visual monitoring of electrophoresis progress (for example, when the 72 kDa band migrates to the lower middle of the gel, it generally indicates sufficient separation), but also effectively distinguishes different molecular weight regions, preventing band confusion. Importantly, the covalent attachment of the dye does not alter the protein's charge or conformation, nor does it affect its normal migration behavior in the gel, ensuring accurate molecular weight determination.3. Ready-to-use formulation: Reduces human error and improves reproducibilityOriscience prestained markers are premixed in 1× SDS-PAGE loading buffer and contain the appropriate amount of glycerol and indicator. No boiling or reducing agents such as β-mercaptoethanol or DTT are required; samples can be loaded directly from the bottle. This design not only simplifies the protocol but also, more importantly, prevents over-reduction, aggregation, or degradation of proteins caused by excessive reducing agents or improper heating, thereby minimizing band tailing and abnormal migration. Furthermore, the standardized buffer system ensures batch-to-batch consistency, significantly improving experimental reproducibility and data reliability.4. Broad molecular weight coverage and clear bands: Precise positioning and aiding transfer verificationThis product covers a molecular weight range of 10–180 kDa and contains 10 distinct bands, making it suitable for molecular weight identification of most target proteins. In Western blot experiments, these pre-stained bands can be visualized after electrophoresis, during transfer, and after transfer, helping researchers to instantly assess transfer completion, uneven transfer, or membrane damage. For example, the 72 kDa orange-red band remains clearly visible on PVDF or nitrocellulose membranes, providing a visual indicator of transfer efficiency and preventing antibody waste and misinterpretation of results due to transfer failure.In today's scientific research, where precision and reproducibility are increasingly sought, a high-quality protein marker is not only a supporting role in experiments but also a key factor in ensuring the authenticity and reliability of data. Oriscience prestained color protein marker effectively avoids the generation of nonspecific bands through high-purity recombinant protein, stable covalent staining, ready-to-use formula and wide-band design, providing a clear, stable and reliable reference system for SDS-PAGE and Western Blot experiments.
