Oriscience Phosphatase Inhibitor 1 Cocktail (100× in ddH2O) is a mixture of 5 phosphatase inhibitors, Sodium Fluoride, Sodium Orthovanadate, Sodium Tartrate Dihydrate, Sodium Molybdate and Imidazole. It can broadly inhibit acid phosphatases, alkaline phosphatases and protein tyrosine phosphatases (PTPs) and is used to maintain the phosphorylation state of proteins. This product can be used for protein immunoassay (WB), immunoprecipitation (IP), co-immunoprecipitation (Co-IP), pull-down, immunohistochemistry (IHC), immunofluorescence (IF), kinase research and other analyses.
Samples such as cell or tissue extracts contain many endogenous protein phosphatases, which can catalyze the dephosphorylation of phosphorylated proteins in the extracts, which is not conducive to the study of phosphorylation differences under physiological and pathological conditions. Therefore, adding appropriate phosphatase inhibitors to samples such as extracts is an effective method to inhibit protein dephosphorylation.
Broad spectrum inhibition - inhibits the activity of multiple phosphatases
Mass spectrometry compatible - does not contain AEBSF, is compatible with mass spectrometry, and is soluble in water
Widely used - can be used for WB, IP, Co-IP, pull-down, IHC, IF, kinase research and other analyses
