PVDF membrane is a polyvinylidene fluoride (PVDF) with a pore size of 0.45 μm. It is most commonly used for transferring proteins from gel to membrane in immunoblotting (Western blotting). It can also be used for double-blotting, adsorption analysis, amino acid analysis, N-terminal protein sequencing, glycoprotein colorimetry, lipopolysaccharide analysis, and mass spectrometry. This hydrophobic PVDF membrane offers excellent protein binding capacity and resolution, low background values, and excellent tensile strength, effectively preventing breakage. Its broad chemical compatibility makes it an ideal transfer membrane for immunoblotting. It contains no charged groups, eliminating electrostatic interactions and exhibiting enhanced protein retention, mechanical strength, and chemical resistance compared to NC membranes. It is recommended for direct protein sequencing and immunoassays after protein electrophoresis (or spot-binding of purified protein samples), particularly for proteins with a molecular weight greater than 20 kDa.
This product is also compatible with a variety of dyes, including Ponceau-S red, Coomassie brilliant blue, Sypro ruby, transillumination, toluidine blue, CPTS, colloidal gold, India ink, and Amido black. After staining with dyes such as Ponceau Red and CPTS, the dye can be washed off, allowing the membrane to be used for further analysis. However, dyes such as Coomassie Brilliant Blue, India Ink, and Amido Black are irreversible, making the membrane unusable for further analysis.
This PVDF membrane is suitable for detection using chemiluminescence (such as Supersensitive ECL and Ultrasensitive ECL), conventional colorimetric methods (such as DAB and TMB), staining, and isotope detection. If fluorescence detection is required (such as with the LI-COR Odyssey system), a dedicated low-fluorescence background PVDF membrane is required.
Wide range of applications – Suitable for chemiluminescence (e.g., ECL), conventional colorimetric development (e.g., DAB, TMB), staining, and isotope detection. High protein binding capacity – Strong protein retention and high binding efficiency. High mechanical strength – Strong stretch resistance, less prone to breakage, and more stable operation. High chemical tolerance – Compatible with organic solvents and suitable for a variety of transfer buffers and staining methods. Excellent staining compatibility – Supports both reversible (e.g., Ponceau Red) and irreversible staining (e.g., Coomassie Brilliant Blue), flexibly meeting subsequent analysis needs.
